University of Maryland School of Medicine  
PKD Core Center
Antibody Validation/Vector Core Mouse Models BioBank Core
Cell Culture Cell Engineering Core Clinical Translational Core

Core Director
Patricia Outeda-Garcia, Ph.D.

Email:
Phone: 410-706-5801


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Genotyping Protocols

PCR Protocol for Genotyping Cre
PCR Protocol for Genotyping Pax8 (Koes)
PCR Protocol for Genotyping PKD1cond
PCR Protocol for Genotyping PKD1KO
PCR Protocol for Genotyping PKD2∧neo (PKD2cond)
PCR Protocol for Genotyping PKD2KO and PKD2∧neo
PCR Protocol for Genotyping PKhD1∧3-4
PCR Protocol for Genotyping Rosa
PCR Protocol for Genotyping SV40 (Immorto) NEW PRIMERS (Kern and Flutcher, 2005)
3 primer genotyping for SV40

The objective of this core is to facilitate the use of mouse models to study Polycystic Kidney Disease.


Mouse models

  • Pkd1 and Pkd2 Knock out alleles in a BL/6 background1,3.
  • Pkd1v/v (knock in GPS cleavage mutant) in a BL/6 background4.
  • Pkhd1del3-4 in a BL/6 background5.
  • Pkd1 and Pkd2 conditional alleles in a BL/6 background1-3
  • Pkd1 and Pkd2 conditional alleles with a reporter and tamoxifen Cre recombinase1-3.
  • Pkd1 and Pkd2 conditional alleles with Pax8-rtTA and TetO-Cre which allows renal tubular inactivation6.
  • Conditional alleles with other Cre recombinase lines available on request.

Biospecimen Repository

  • Tissue specimens from Pkd1 null and Pkd2 null animals at E10.5, E12.5, E14.5
  • Tissue specimens, blood and urine from Pkd1cond/cond; tamoxifen Cre, Rosa (and controls) induced at P212
  • Specimens collected monthly post induction, correlated with ultrasound.
  • Samples available from additional models upon request

Expertise

  • Histopathology
  • Model development and characterization
  • Assistance with design of efficient breeding strategies
  • Preclinical therapeutic trial assistance for research base members on a limited basis

References

  1. Piontek KB et al. A functional floxed allele of Pkd1 that can be conditionally inactivated in vivo. J Am Soc Nephrol. 2004; 15: 3035-43.
  2. Piontek KB et al. A critical developmental switch defines the kinetics of kidney cyst formation after loss of Pkd1. Nature Medicine. 2007; 13: 1490-5.
  3. Garcia-Gonzalez MA et al. Pkd1 and Pkd2 are required for normal placental development. PLoS One. 2010; 5, issue 9.
  4. Yu S, Hackmann K, Gao J et al. Essential role of cleavage of Polycystin-1 at G protein-coupled receptor proteolytic site for kidney tubular structure. Proc Natl Acad Sci USA. 2007; 104:18688-93.
  5. Garcia-Gonzalez MA et al. Genetic interaction studies link autosomal dominant and recessive polycystic kidney disease in a common pathway. Hum Mol Genet. 2007; 16:1940-50.
  6. Traykova-Brauch M et al. An efficient and versatile system for acute and chronic modulation of renal tubular function in transgenic mice. Nat Med. 2008; 14: 979-84.
Eduational Programs Pilot & Feasibility Program

The University of Maryland Medical Center and the Division of Nephrology provide multidisciplinary care for patients with PKD. For Appointments call 410-328-5720 and ask for Karleen Schuhart. Make an appointment.

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