University of Maryland School of Medicine  
PKD Core Center
Antibody Validation/Vector Core Mouse Models BioBank Core
Cell Culture Cell Engineering Core Clinical Translational Core

Core Director
Patricia Outeda-Garcia, Ph.D.

Phone: 410-706-5801

Request Reagents

Genotyping Protocols

PCR Protocol for Genotyping Cre
PCR Protocol for Genotyping Pax8 (Koes)
PCR Protocol for Genotyping PKD1cond
PCR Protocol for Genotyping PKD1KO
PCR Protocol for Genotyping PKD2∧neo (PKD2cond)
PCR Protocol for Genotyping PKD2KO and PKD2∧neo
PCR Protocol for Genotyping PKhD1∧3-4
PCR Protocol for Genotyping Rosa
PCR Protocol for Genotyping SV40 (Immorto) NEW PRIMERS (Kern and Flutcher, 2005)
3 primer genotyping for SV40

The objective of this core is to facilitate the use of mouse models to study Polycystic Kidney Disease.

Mouse models

  • Pkd1 and Pkd2 Knock out alleles in a BL/6 background1,3.
  • Pkd1v/v (knock in GPS cleavage mutant) in a BL/6 background4.
  • Pkhd1del3-4 in a BL/6 background5.
  • Pkd1 and Pkd2 conditional alleles in a BL/6 background1-3
  • Pkd1 and Pkd2 conditional alleles with a reporter and tamoxifen Cre recombinase1-3.
  • Pkd1 and Pkd2 conditional alleles with Pax8-rtTA and TetO-Cre which allows renal tubular inactivation6.
  • Conditional alleles with other Cre recombinase lines available on request.

Biospecimen Repository

  • Tissue specimens from Pkd1 null and Pkd2 null animals at E10.5, E12.5, E14.5
  • Tissue specimens, blood and urine from Pkd1cond/cond; tamoxifen Cre, Rosa (and controls) induced at P212
  • Specimens collected monthly post induction, correlated with ultrasound.
  • Samples available from additional models upon request


  • Histopathology
  • Model development and characterization
  • Assistance with design of efficient breeding strategies
  • Preclinical therapeutic trial assistance for research base members on a limited basis


  1. Piontek KB et al. A functional floxed allele of Pkd1 that can be conditionally inactivated in vivo. J Am Soc Nephrol. 2004; 15: 3035-43.
  2. Piontek KB et al. A critical developmental switch defines the kinetics of kidney cyst formation after loss of Pkd1. Nature Medicine. 2007; 13: 1490-5.
  3. Garcia-Gonzalez MA et al. Pkd1 and Pkd2 are required for normal placental development. PLoS One. 2010; 5, issue 9.
  4. Yu S, Hackmann K, Gao J et al. Essential role of cleavage of Polycystin-1 at G protein-coupled receptor proteolytic site for kidney tubular structure. Proc Natl Acad Sci USA. 2007; 104:18688-93.
  5. Garcia-Gonzalez MA et al. Genetic interaction studies link autosomal dominant and recessive polycystic kidney disease in a common pathway. Hum Mol Genet. 2007; 16:1940-50.
  6. Traykova-Brauch M et al. An efficient and versatile system for acute and chronic modulation of renal tubular function in transgenic mice. Nat Med. 2008; 14: 979-84.
Eduational Programs Pilot & Feasibility Program

The University of Maryland Medical Center and the Division of Nephrology provide multidisciplinary care for patients with PKD. For Appointments call 410-328-5720 and ask for Karleen Schuhart. Make an appointment.

Copyright © 2013 Baltimore PKD Core Center. All Rights Reserved. Site designed by Academic Web Pages.